As others have pointed out, the femme fatale definition is quite hard to fill, even the badass women are a rarity. From the little I've seen, Bayonetta definitely fits that description.
But if I'm going with a character from a game I've played, I'm going to go with Gorgo, Queen of Sparta, from Civ VI; you know she doesn't mess around when she throws down her spear and belittles you for not warring enough: "Are you such a coward, to avoid bloodshed? To yield so easily? Where is your honor? "