No audio output from audio jacks

My motherboard is gigabyte H97 D3H

I was using my 2.1 speaker which has 3.5 mm jack and it was plugged into rear green audio jack and it was working fine for atleast 10 months without any issues and but suddenly my speakers aren't working and I also changed speakers to test but still there is no output. I have tried uninstalling and reinstalling and updating audio driver it didn't worked.

I am not using front port as I have removed it's connection from motherboard.
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