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Can single gpu be placed in 2nd PCIe slot on Asus Strix B350-F Gaming Motherboard

I am hooking up my Asus Strix 1060-O6D with Ryzen 7 1700 on Asus B350-F Motherboard with ADATA D40 2666Mhz 8GBx2 Ram.

Now Just wanted to know that is it possible to hook my 1060 in lower PCIe lanes as my mobo consists of 3 PCIe x16 lanes. Will there be any performance difference or should i have to stick with the top one.
Reply to Nikhil_sood
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More about single gpu 2nd pcie slot asus strix b350 gaming motherboard
  1. If they are all full speed x16 no problems.
    Some motherboards cut bandwidth to the second slot. Check manual. If all are x16 you can choose whichever you want.
    Reply to R_1
  2. Best answer
    Nikhil_sood said:
    is it possible to hook my 1060 in lower PCIe lanes. Will there be any performance difference or should i have to stick with the top one.

    Possible -yes. Performance difference - also yes.

    For single GPU it's recommended to use upper PCIE x16_1 slot.
    Lower PCIE x16 slots will operate at lower performance modes. When using PCIE x16_2 slot, slot configuration will switch to x8/x4 mode. PCIE x16_3 slot operates at x4 mode max.

    Read motherboard manual.
    https://www.asus.com/Motherboards/ROG-STRIX-B350-F-GAMING/HelpDesk_Manual/
    Reply to SkyNetRising
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